100% secure
Email this product page
Print this page

IRAK4 Antibody

IRAK4 Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

  • IRAK4 Antibody thumbnail image 1
  • Goat anti IRAK4 (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1098E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti IRAK4 antibody detects an epitope within the N-terminal (NT) region of integrin-1 receptor-associated kinase 4 (IRAK4). IRAK4 has a molecular weight of ~50 kDa, and is an important mediator in the signal transduction of Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor family members. IRAK4 functions in this signal transduction pathway by binding to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signalling cascades leading to transcriptional up-regulation and mRNA stabilization.

      IRAK4 is a key activator of TLR-mediated signal transductions and has crucial roles in innate immune responses. It is expressed by the liver, kidneys and skeletal muscle.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ChimpanzeeExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human IRAK4 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence NKPITPSTYVRC corresponding to the N-terminal of IRAK4 (NP_057207.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      toll-like receptor 2 signaling pathway
      protein phosphorylation
      endosome membrane
      magnesium ion binding
      plasma membrane
      ATP binding
      MyD88-dependent toll-like receptor signaling pathway
      toll-like receptor 4 signaling pathway
      Toll signaling pathway
      innate immune response
      protein serine/threonine kinase activity
      toll-like receptor 1 signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting13ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1098 detects a band of approximately 50kDa in A431 and HeLa cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
    • Instructions For Use

    Additional IRAK4 Antibody Formats

    Formats Applications Sizes available
    IRAK4 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Write your review

              You may also be interested in...