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IRAK1 antibody

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Anti Human IRAK1 (C-Terminal) Antibody thumbnail image 1 Anti Human IRAK1 (C-Terminal) Antibody thumbnail image 2
Anti Human IRAK1 (C-Terminal) Antibody gallery image 1
Western Blot analysis of THP-1 (THP) and HeLa (HL) cell lysates probed with Rabbit anti Human IRAK-1 (AHP445)
Anti Human IRAK1 (C-Terminal) Antibody gallery image 2
Immunocytochemical staining of HeLa cells with Rabbit anti Human IRAK-1 (AHP445)

Rabbit anti Human IRAK1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IRAK1
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP445
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IP WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Human IRAK1 antibody recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain and a single death domain. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide from C terminus of human IRAK
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation 2-4ug/sample
Western Blotting 1/1000 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Cao, Z. et al. (1996) IRAK: a kinase associated with the interleukin-1 receptor.
    Science. 271 (5252): 1128-31.

RRID
AB_322748
UniProt
P51617
Entrez Gene
IRAK1
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0001959 regulation of cytokine-mediated signaling pathway
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004704 NF-kappaB-inducing kinase activity
GO:0004842 ubiquitin-protein ligase activity
GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
GO:0005524 ATP binding
GO:0006916 anti-apoptosis
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0016563 transcription activator activity
GO:0031663 lipopolysaccharide-mediated signaling pathway
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042803 protein homodimerization activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0045323 interleukin-1 receptor complex
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051259 protein oligomerization
GO:0051403 stress-activated MAPK cascade
GO:0070498 interleukin-1-mediated signaling pathway
GO:0070555 response to interleukin-1

AHP445

060514 290414

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