IQGAP1 Antibody (PrecisionAbTM Antibody)

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IQGAP1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with IQGAP1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 43 second exposure. Arrow points to IQGAP1 (molecular weight 189 kDa)

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  • IQGAP1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti IQGAP1
  • IQGAP1 Antibody with Control Lysate
  • IQGAP1 Antibody with Control Lysate
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    IQGAP1 antibody recognizes Ras GTPase-activating-like protein IQGAP1, also known as p195. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00054WBdatasheet pdfdatasheet pdf100 µl
      VPA00054
      VPA00054KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00054K
      VPA00054KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00054KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human IQGAP1 antibody recognizes human IQGAP1, a member of the IQGAP family. IQGAP1 is scaffold protein of, among others, the MAPK pathway, binding three consecutive signaling molecules RAF, MEK and ERK and thereby facilitating their interaction. Through this role in a core signaling network, IQGAP has major impact on cytoskeletal reorganization, proliferation, cell motility and invasion (White et al., 2012).

        Researchers have identified IQGAP1 as important to the hyperactive MAPK signaling associated with several forms of cancer ( Jameson et al., 2013).

        Goat anti Human IQGAP1 detects IQGAP1 as a band of 189 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
      • Target Species
        Human
      • Western Blotting
        Goat anti IQGAP1 detects a band of approximately 189 kDa in HeLa cell lysates.
        Goat anti IQGAP1 detects a band of approximately 189 kDa in HeLa cell lysates.
        Goat anti IQGAP1 detects a band of approximately 189 kDa in HeLa cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-KRQSGQTDPLQKEE, from the internal region of the protein sequence.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        calmodulin binding
        regulation of small GTPase mediated signal transduction
        GTPase inhibitor activity
        plasma membrane
        Ras GTPase activator activity
        small GTPase mediated signal transduction
        regulation of insulin secretion
        midbody
        energy reserve metabolic process
        cytoplasm
        protein phosphatase binding
        actin filament
        nucleus
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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