IP-10 Antibody

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IP-10 Antibody gallery image 1

Formalin fixed, paraffin embedded human carcinoid tissue stained with Rabbit anti Human IP-10 (AHP782) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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IP-10 Antibody gallery image 2

Formalin fixed, paraffin embedded human carcinoid tissue stained with Rabbit anti Human IP-10 (AHP782) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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IP-10 Antibody gallery image 3

Formalin fixed, paraffin embedded human carcinoid tissue stained with Rabbit anti Human IP-10 (AHP782) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Enlarge
IP-10 Antibody gallery image 4

Formalin fixed, paraffin embedded human carcinoid tissue stained with Rabbit anti Human IP-10 (AHP782) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Enlarge
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  • Rabbit anti Human IP-10
  • Rabbit anti Human IP-10:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP782E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP782
    AHP782BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP782B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human IP-10 antibody recognizes human IP-10 (10-kDa interferon-inducible protein), otherwise known as CXCL10, a 77 amino acid CXC chemokine which, like monokine induced by IFN-gamma (Mig) and IFN-inducible T-cell alpha-chemoattractant (I-TAC), signals through the CXCR3 receptor.

      IP-10 acts as a chemoattractant for Th1 lymphocytes and natural killer (NK) cells and is secreted by a variety of cells, including neutrophils, monocytes, keratinocytes and epithelial cells, in response to interferon gamma (IFNgamma) and lipopolysaccharide (LPS). IP-10 is thus associated with several clinical conditions linked with Th1 type immune responses and raised IFNgamma expression, such as psoriasis, sarcoidosis and viral meningitis. In addition, studies have demonstrated the ability of IP-10 to suppress the synergistic action of stimulatory cytokines on hematopoietic progenitor cell proliferation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human IP-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present.
      None present.
    • Immunogen
      Recombinant human IP-10.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml after reconstitution
      IgG concentration 0.1 mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      cell surface receptor linked signaling pathway
      blood circulation
      chemotaxis
      inflammatory response
      positive regulation of cell proliferation
      muscle organ development
      chemokine activity
      extracellular space
      cell-cell signaling
      cAMP-dependent protein kinase regulator activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)1.0ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human IP-10 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IP-10 antibody (AHP782B) as the detection antibody
    • ELISA
      This biotinylated human IP-10 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IP-10 antibody (AHP782) as the capture antibody.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IP-10 Antibody Formats

    Formats Applications Sizes available
    IP-10 Antibody : Biotin E, WB 50 µg
    IP-10 Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Recommended Positive Controls

            Histology Controls

              Further Reading

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