Inhibin Beta B, otherwise known as Activin B, is a member of the transforming growth factor-beta (TGF-B) family, which signals through the ActRII receptor (Activin Receptor type II), and acts as a regulator of hormone secretion from the pituitary, hypothalamic and gonadal glands.
Inhibin Beta B is a homodimer of the inhibin/activin beta-B chain, and is involved in several aspects of embryonic development, as well as haematopoiesis and osteogenesis, and also acts as a modulator of FSH (follicle-stimulating hormone) secretion and haemoglobin synthesis.
Purified recombinant protein - lyophilised
Reconstitute with 10ul 10mM sodium citrate pH 3,0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant Inhibin Beta B expressed in High-5 insect cells
The ED50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is = 2ng/ml, corresponding to a specific activity of = 5 x 105 units/mg.
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.5 mg/ml after reconstitution
25.6kD (115 amino acid Beta B homodimer)
Reagents In The Kit
Preparing The Antibody
10mM Sodium citrate.
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.