Inhibin Alpha Antibody | 5070

//www.bio-rad-antibodies.com/human-inhibin-alpha-antibody-5070-hca070.html?RenderPageSpecific=1
Inhibin Alpha Antibody | 5070 gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • Inhibin Alpha Antibody | 5070 thumbnail image 1
  • Human anti Human Inhibin Alpha
  • Human anti Human Inhibin Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5070
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA070C, E, P *datasheet pdfdatasheet pdf0.1 mg
    HCA070
    HCA070AC, E, P *datasheet pdfdatasheet pdf50 µg
    HCA070A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Inhibin alpha antibody, clone 5070 specifically recognizes the 32kDa alpha subunit of human inhibin.

      Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumors, placental and gestational trophoblastic lesions and sex cord stromal tumors.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Peptide comprising the first 29 amino acids of the mature Inhibin Alpha chain (amino acids 233-262).
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml.
      Pack Size: 50 µgAntibody concentration 0.1mg/ml following reconstitution
      Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml.
      Pack Size: 50 µgAntibody concentration 0.1mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
      response to external stimulus
      cell surface receptor linked signaling pathway
      signal transducer activity
      skeletal system development
      negative regulation of interferon-gamma biosynthetic process
      cell cycle arrest
      negative regulation of phosphorylation
      negative regulation of B cell differentiation
      negative regulation of follicle-stimulating hormone secretion
      negative regulation of macrophage differentiation
      inhibin A complex
      growth factor activity
      ovarian follicle development
      cytokine activity
      hemoglobin biosynthetic process
      induction of apoptosis
      erythrocyte differentiation
      inhibin-betaglycan-ActRII complex
      regulation of cell proliferation
      nervous system development
      positive regulation of follicle-stimulating hormone secretion
      hormone activity
      cell-cell signaling
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Inhibin Alpha Antibody Formats

    Formats Clone Applications Sizes available
    Inhibin Alpha Antibody : Purified 5070 C, E, P * 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...