Indoleamine 2,3-Dioxygenase Antibody

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  • Sheep anti Human Indoleamine 2,3-Dioxygenase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP833P *, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP833
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Human Indoleamine 2,3-Dioxygenase antibody recognises human indoleamine 2,3-dioxygenase (IDO), an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. Reports suggest that IDO is involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Marmosetyes
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ig Fraction - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human indoleamine dioxygenase (IDO) were raised by repeated immunisation of sheep with highly purified antigen. Ig fraction prepared by ammonium sulphate precipitation.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant human indoleamine 2,3-dioxygenase.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 27.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      oxidation-reduction process
      electron carrier activity
      tryptophan 2,3-dioxygenase activity
      indoleamine 2,3-dioxygenase activity
      female pregnancy
      heme binding
      cytosol
      tryptophan catabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1/1000
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Indoleamine 2,3-Dioxygenase Antibody Formats

    Formats Applications Sizes available
    Indoleamine 2,3-Dioxygenase Antibody : Ig Fraction P *, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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