Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD11b (MCA551A700) and FITC conjugated anti Human IL-8 (MCA2790F). Figure B. Cells stimulated with LPS for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD11b (MCA551A700) and FITC conjugated Mouse anti Human IL-8 (MCA2790F). All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer
Mouse anti Human Interleukin-8 antibody, clone NAPII human IL-8 (interleukin-8), a pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils, T cells and basophils.
IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G
0.02% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.