IL-7 Antibody | BVD10-11C10

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Sandwich ELISA analysis of human interleukin-7 using Rat anti Human IL-7 (1012201) as a capture reagent and biotinylated Rat anti Human IL-7 (1012308) as a detection reagent with recombinant human IL-7 (PHP046) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A human serum sample is indicated (green)

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  • Rat anti Human Interleukin-7:Biotin
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1012308Edatasheet pdfdatasheet pdf0.5 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Rat anti Human Interleukin-7 antibody, clone BVD10-11C10 recognizes human interleukin 7 (IL-7), also known as Lymphopoietin 1 or pre-B cell factor. IL-7 is a 177 amino acid haematopoietic growth factor stimulating primarily early B and T lymphocytes. IL- 7 is required for development of T-cells in the thymus (Plum et al. 1996) and absence of the IL-7 receptor α chain (CD127) leads to a severe combined immunodeficiency condition through the lack of T-cell production (Roifman et al. 2000). IL-7 is also important post-thymically for homeostasis of naive T-cell populations and generation and survival of CD4+ memory T-cells (Kondrack et al. 2003).

      IL-7 deficiency or deficient function is also noted in immunodeficiency diseases including rheumatoid arthritis (Leonard 2005), chronic colitis (Totsuka et al. 2007). multiple sclerosis through CD127 (Svejgaard 2008) and diabetes (Li et al. 2011).

      Rat anti Human Interleukin-7 antibody, clone BVD10-11C10 in biotinylated format has been used sucessfully in the development of a sensitive ELISA assay as a detection reagent for human IL-7. It has been shown to recognize both native and recombinant human IL-7, in conjunction with clone BVD10-40F6 as a capture reagent.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with 1012201 as the capture reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-7 Antibody Formats

    Formats Clone Applications Sizes available
    IL-7 Antibody : Biotin BVD10-11C10 E 0.5 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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