IL-7 antibody
Rabbit anti Human Interleukin-7:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-7
Rabbit anti Human Interleukin-7 antibody recognizes human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human IL-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-7 (PHP046).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.15 | 0.30ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
- ELISA
- Rabbit anti Human IL-7 (biotin conjugate) may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with recombinant human IL-7 as the standard and Rabbit anti Human IL-7 (purified) as the capture reagent.
References for IL-7 antibody
-
Lupton, S.D. et al. (1990) Characterization of the human and murine IL-7 genes.
J Immunol. 144 (9): 3592-601. -
Fry, T.J. & Mackall, C.L. (2002) Interleukin-7: from bench to clinic.
Blood. 99 (11): 3892-904.
- RRID
- AB_2233607
- UniProt
- P13232
- Entrez Gene
- IL7
- GO Terms
- GO:0005125 cytokine activity
- GO:0005139 interleukin-7 receptor binding
- GO:0005615 extracellular space
- GO:0006959 humoral immune response
- GO:0007267 cell-cell signaling
- GO:0009887 organ morphogenesis
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0045453 bone resorption
- View More GO Terms
- GO:0045582 positive regulation of T cell differentiation
AHP283B
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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