IL-4 Antibody

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Staining of fomalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human Interleukin-4 (AHP780) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • IL-4 Antibody thumbnail image 1
  • Rabbit anti Human Interleukin-4:Biotin
  • Rabbit anti Human Interleukin-4
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP780E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP780
    AHP780BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP780B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Interleukin-4 antibody recognizes human IL-4 (Interleukin-4), also known as B-cell stimulatory factor 1, a 129 amino acid pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching.

      IL-4 signalling through the IL-4 receptor (IL-4R), under the influence of Stat6, induces the differentiation of CD4+ T cells into Th2 helper cells, which themselves then secrete IL-4 along with IL-5, IL-6, IL-10 and IL-13. Excessive secretion of IL-4 by Th2 helper cells has been linked with allergy and elevated IgE levels.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
    • Preparation
    • Antiserum Preparation
      Antisera to human IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml after reconstitution.
      IgG concentration 1.0mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      interleukin-4 receptor binding
      B cell differentiation
      positive regulation of MHC class II biosynthetic process
      cholesterol metabolic process
      positive regulation of isotype switching to IgG isotypes
      positive regulation of B cell proliferation
      positive regulation of transcription from RNA polymerase II promoter
      connective tissue growth factor biosynthetic process
      negative regulation of apoptosis
      transcription activator activity
      negative regulation of osteoclast differentiation
      growth factor activity
      extracellular space
      chemotaxis
      cytokine activity
      positive regulation of isotype switching to IgE isotypes
      cellular defense response
      type 2 immune response
      transcription repressor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays0.030.05ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human IL-4 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-4 antibody (AHP780) as the capture reagent and recombinant human IL-4 (PHP234) as the standard.
    • ELISA
      This purified human IL-4 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IL-4 antibody (AHP780B) as the detection reagent and recombinant human IL-4 (PHP234) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-4 Antibody Formats

    Formats Applications Sizes available
    IL-4 Antibody : Purified E, FN, P *, WB 0.1 mg
    IL-4 Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR121B
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
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      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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