IL-33 Antibody | 7786

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IL-33 Antibody | 7786 gallery image 1

Sandwich ELISA with Human anti Human IL-33 (HCA140) as coating anitbody; human IL-33 as antigen and Rabbit anti Human Interleukin-33:Biotin (AHP1482B) as detection antibody;followed by Streptavidin:HRP (STAR5B)

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IL-33 Antibody | 7786 gallery image 2

Sandwich ELISA with Human anti Human IL-33 (HCA140) as coating anitbody; human IL-33 as antigen and Rabbit anti Human Interleukin-33:Biotin (AHP1482B) as detection antibody;followed by Streptavidin:HRP (STAR5B)

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  • Human anti Human Interleukin-33
  • Human anti Human Interleukin-33
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    7786
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA140Edatasheet pdfdatasheet pdf0.1 mg
    HCA140
    HCA140AEdatasheet pdfdatasheet pdf50 µg
    HCA140A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Interleukin-33 antibody, clone 7786 specifically recognizes Human Interleukin-33 (IL-33), a cytokine formerly known as NF-HEV (Nuclear factor from high endothelial venules).

      IL-33 belongs to the IL-1 super family which signals through interaction with the ST2 and Toll-interleukin 1 (IL-1) receptors, and is known to act as an immunoregulator of T helper 2 (Th2) cell cytokine synthesis.

      IL-33 is abundantly expressed in high endothelial venules and found in tonsils, peyers patches and mesenteric lymph nodes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0 ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0 ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Human IL33-N1 fusion protein.
    • Purity
    • Approx. Protein Concentrations
      0.1 mg/ml
      0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of chemokine secretion
      positive regulation of inflammatory response
      extracellular space
      cytokine activity
      positive regulation of transcription from RNA polymerase II promoter
      positive regulation of macrophage activation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP1482B as the detection reagent.
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP1482B as the detection reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-33 Antibody Formats

    Formats Clone Applications Sizes available
    IL-33 Antibody : Purified 7786 E 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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