Human interleukin-3 is an E. coli-derived recombinant protein corresponding to full-length mature human interleukin-3. Interleukin-3 supports the formation of multilineage colonies in the early development of multipotent haematopoietic progenitor cells. It has been shown to induce colony formation of macrophages, neutrophils, mast cells and megakaryocytes from agar-suspended bone marrow cells.
Purified recombinant protein - lyophilised
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Recombinant protein expressed in E. coli.
>1 x 107 units/mg
>98% by SDS PAGE analysis and N terminal sequence analysis
Approx. Protein Concentrations
Protein Molecular Weight
15kDa (133 amino acids)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.