100% secure
Email this product page
Print this page


IL-3 gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

  • IL-3 thumbnail image 1
  • Recombinant Human Interleukin-3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP043FNdatasheet pdfdatasheet pdf10 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Human interleikin-3 is an E. coli-derived recombinant protein corresponding to full-length mature human interleukin-3. Interleikin-3 supports the formation of multilineage colonies in the early development of multipotent haematopoietic progenitor cells. It has been shown to induce colony formation of macrophages, neutrophils, mast cells and megakaryocytes from agar-suspended bone marrow cells.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
    • Preparation
      Recombinant protein expresed in E. coli.
    • Preservative Stabilisers
      None present
    • Activity
      >1 x 107 units/mg
    • Purity
      >98% by SDS PAGE analysis and N terminal sequence analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      15kDa (133 amino acids)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      cytokine activity
      positive regulation of tyrosine phosphorylation of Stat5 protein
      nervous system development
      growth factor activity
      interleukin-3 receptor binding
      extracellular space
      positive regulation of survival gene product expression
      positive regulation of cell proliferation
      cell-cell signaling
      immune response
      positive regulation of DNA replication
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.1ng/ml1.0ng/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-3 Formats

    Formats Applications Sizes available
    IL-3 : Rec. Protein FN 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...