IL-27 Alpha Antibody | H08-5C8

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  • Mouse anti Human Interleukin 27 Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    H08-5C8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5965GAEdatasheet pdfdatasheet pdf0.1 mg
    MCA5965GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human IL-27α, clone H08-5C8, recognizes IL-27α, a subunit of IL-27. IL-27 is a 62 kDa member of the Il-6/IL-12 family of long type I cytokines. IL-27 is heterodimeric group 2 receptor composed of Epstein-Barr Virus induced gene 3 (EBI3) and IL-27α. EBI3 is a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23 and IL-27α is a 28 kDa glycoprotein that is related to the p35 of chain IL-12.

      IL-27 has broad range of pro- and anti-inflammatory properties, mediating its effects via its gp130-associated cytokine receptor and JAK/STAT signalling cascades. It can alter T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells and has diverse effects on innate immune cells. In vivo, it is reported to play an important role in immune regulation, as mice with defects in IL-27 or its receptor display enhanced immune responses (Batten & Ghilardi 2007).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from ascites
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      NS0-derived recombinant human IL-27
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      regulation of T cell proliferation
      cytokine activity
      positive regulation of interferon-gamma biosynthetic process
      regulation of T-helper 1 cell differentiation
      innate immune response
      regulation of defense response to virus
      interleukin-27 receptor binding
      inflammatory response
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-27 Alpha Antibody Formats

    Formats Clone Applications Sizes available
    IL-27 Alpha Antibody : Purified H08-5C8 E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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