Interleukin-22 (IL-22) is a regulatory cytokine and member of the IL-10 family, secreted by activated T cells, which signals through a class II cytokine receptor system consisting of IL-22R and IL-10R-beta/CRF2-4.
IL-22 has been shown to act as an inhibitor of the production of IL-4 by Th2 cells, up-regulate the production of acute phase reactants in the pancreas and liver, and to be up-regulated in intestinal inflammation associated with Crohn’s disease.
Purified recombinant protein - lyophilised
Reconstitute with 100ul distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant IL-22 expressed in E.coli
Determined by its ability to activate STAT following receptor ligand interaction.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
33.6kD (146 amino acid homodimer)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.