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IL-22 antibody

Rabbit anti Human Interleukin-22

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-22

Product Code Applications Pack Size List Price Your Price Qty
AHP1789
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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loader

Rabbit anti Human Interleukin-22 antibody specifically recognizes human interleukin-22 (IL-22), a regulatory cytokine and member of the IL-10 family, secreted by activated T cells, which signals through a class II cytokine receptor system consisting of IL-22R and IL-10R-beta/CRF2-4.

IL-22 has been shown to act as an inhibitor of the production of IL-4 by Th2 cells, up-regulate the production of acute phase reactants in the pancreas and liver, and to be up-regulated in intestinal inflammation associated with Crohn’s disease.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antiserum to human IL-22 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 1.0 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human IL-22 antibody may be used in an indirect ELISA with recombinant human IL-22 (PHP209) as the standard.

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Synonyms
IL-TIF
RRID
AB_2264692
UniProt
Q9GZX6
Entrez Gene
IL22
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007267 cell-cell signaling
GO:0045518 interleukin-22 receptor binding

AHP1789

150441 157677 158498 161691 165012

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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