IL-1RA Antibody (PrecisionAbTM Antibody)

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IL-1RA Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with IL-1RA antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 1 second exposure. Arrow points to IL-1RA (molecular weight 18 kDa)

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  • IL-1RA Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti Interleukin-1RA
  • Interleukin-1RA ANTIBODY with Control Lysate
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    Mouse anti-human IL-1RA antibody recognizes interleukin-1 receptor antagonist protein, also known as IL-1 inhibitor, IL-1RN (IL-1F3) or intracellular IL-1 receptor antagonist type II. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00500WBdatasheet pdfdatasheet pdf100 µl
      VMA00500
      VMA00500KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00500KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human interleukin-1RA antibody recognizes the interleukin-1 receptor antagonist protein, also known as IL1 inhibitor, intracellular interleukin-1 receptor antagonist or type II interleukin-1 receptor antagonist.

        The protein encoded by IL1RN gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL1RN and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of IL1RN is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

        Mouse anti Human interleukin-1RA antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti interleukin-1RA detects a band of approximately 18 kDa in A431 cell lysate
        Anti IL-1RA detects a band of approximately 18 kDa in A431 cell lysate
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E. coli derived recombinant protein corresponding to amino acids 26-177 of human interleukin-1RA (IL-1RA)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        immune response
        inflammatory response
        centrosome
        response to glucocorticoid stimulus
        nucleus
        plasma membrane
        extracellular space
        cytokine activity
        interleukin-1 receptor antagonist activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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