IL-17 Antibody

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Staining of formalin fixed,paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-17 (AHP455G)

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IL-17 Antibody gallery image 2

Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Goat anti Human IL-17A (AHP455G) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0)

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP455GE, FN*, IF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP455G
    AHP455BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP455B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Interleukin-17 antibody recognizes human IL-17 (Interleukin-17/17A), a 32kDa disulfide-linked homodimer consisting of two 136 amino acid chains, which belongs to the IL-17 family (classified as IL-17A to IL-17F), and signals through the IL-17 receptor (IL-17R/CDw217).

      IL-17 is produced primarily by activated CD4+ T cells and induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNFalpha, IFNgamma and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. The role of IL-17 in bone metabolism has become a focus area for studies into Rheumatoid Arthritis, in which levels of IL-17 are significantly increased and may contribute to bone destruction.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Antiserum Preparation
      Antisera to human IL-17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
    • Preservative Stabilisers
      None present.
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml after reconstitution
      IgG concentration 1.0 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      cytokine activity
      positive regulation of osteoclast differentiation
      positive regulation of transcription from RNA polymerase II promoter
      extracellular space
      immune response
      cell-cell signaling
      apoptosis
      protein glycosylation
      inflammatory response
      positive regulation of interleukin-23 production
      cell activation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Immunofluorescence
      Western Blotting0.10.2ug/ml
      Functional Assays(1)0.931.3ug/ml
      Immunohistology - Paraffin(2)0.25ug/ml
      (1)
      To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml).
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human IL-17 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-17 antibody (AHP455G) as the capture reagent and recombinant human IL-17 (PHP098) as the standard.
    • ELISA
      This purified human IL-17 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IL-17 antibody (AHP455B) as the detection reagent and recombinant human IL-17 (PHP098) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-17 (PHP098) as the positive control.
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-17 (PHP098) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional IL-17 Antibody Formats

    Formats Applications Sizes available
    IL-17 Antibody : Purified E, FN*, IF, P *, WB 0.1 mg
    IL-17 Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-16AHP454G0.1 mgE, FN, WB
          AHP454G
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Human Interleukin-17:BiotinAHP455B50 µgE, WB
          AHP455B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interleukin-17PHP09825 µgE, FN, WB
            PHP098
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interleukin-17PHP09825 µgE, FN, WB
            PHP098

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

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