This product is an E. coli-derived recombinant protein corresponding to full-length mature human Interleukin-15 (IL-15), a cytokine that stimulates proliferation of T-lymphocytes
Purified recombinant protein - lyophilised.
Reconstitute with 20 ul distilled water. For extended storage the addition of 0.1% BSA and 0.09% sodium azide is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. N.B. For functional studies do not add sodium azide
PHP099A has a specific activity of > 2 x 106 units/mg as determined by dose-dependent proliferation assay of CTLL-2 cells.
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml after reconstitution
Protein Molecular Weight
12.9 kDa based on 115 amino acid sequence
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.