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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP099AFNdatasheet pdfdatasheet pdf10 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • This product is an E. coli-derived recombinant protein corresponding to full-length mature human Interleukin-15 (IL-15), a cytokine that stimulates proliferation of T-lymphocytes
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised.
    • Reconstitution
      Reconstitute with 20 ul distilled water. For extended storage the addition of 0.1% BSA and 0.09% sodium azide is recommended.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Preservative Stabilisers
      None present
    • Activity
      PHP099A has a specific activity of > 2 x 106 units/mg as determined by dose-dependent proliferation assay of CTLL-2 cells.
    • Purity
      >98% by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml after reconstitution
    • Protein Molecular Weight
      12.9 kDa based on 115 amino acid sequence
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      Sodium Phosphate
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      cytokine receptor binding
      positive regulation of interleukin-17 production
      immune response
      cytokine activity
      signal transducer activity
      extracellular space
      positive regulation of tissue remodeling
      cell-cell signaling
      membrane fraction
      positive regulation of inflammatory response
      Golgi apparatus
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.1ng/ml10ng/ml

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-15 Formats

    Formats Applications Sizes available
    IL-15 : Rec. Protein FN 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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