IL-1 Alpha

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  • Recombinant Human Interleukin-1 Alpha
  • Recombinant Human Interleukin-1 Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP040AFNdatasheet pdfdatasheet pdf10 µg
    PHP040A
    PHP040FNdatasheet pdfdatasheet pdf2 µg
    PHP040
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human Interleukin - 1alpha is a potent immunomodulatory protein, mediating a range of inflammatory processes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
      Purified recombinant protein - lyophilised
    • Reconstitution
      Pack Size: 10 µgReconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 2 µgReconstitute with 20 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 10 µgReconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 2 µgReconstitute with 20 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein prepared from E. coli.
      Recombinant protein prepared from E. coli.
    • Preservative Stabilisers
      None present
      None present
    • Activity
      1 x 109 units/mg
    • Purity
      >98 % by SDS PAGE and HPLC analysis
      >98 % by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml
      0.1mg/ml
    • Protein Molecular Weight
      18kD (159 amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1ng/ug
    • Test Principle
    • Buffer Solution
      Tris buffered saline
      Tris buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
      3 months from date of reconstitution.
    • GO Terms
      apoptosis
      cytosol
      positive regulation of cytokine secretion
      copper ion binding
      anti-apoptosis
      cytokine activity
      negative regulation of cell proliferation
      response to copper ion
      positive regulation of interleukin-2 biosynthetic process
      interleukin-1 receptor binding
      immune response
      positive regulation vascular endothelial growth factor production
      positive regulation of mitosis
      extracellular space
      cytokine-mediated signaling pathway
      positive regulation of cell division
      cell proliferation
      positive regulation of angiogenesis
      fever generation
      cellular response to heat
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.1ng/ml10ng/ml
    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.1ng/ml10ng/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Alpha Formats

    Formats Applications Sizes available
    IL-1 Alpha : Rec. Protein FN 10 µg | 2 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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