IL-1 Alpha Antibody

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IL-1 Alpha Antibody gallery image 1

Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human IL-1β (AHP281) following enzume mediated antigen retrieval using proteinase K

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IL-1 Alpha Antibody gallery image 2

Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human IL-1β (AHP281) following enzume mediated antigen retrieval using proteinase K

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IL-1 Alpha Antibody gallery image 3

Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human IL-1β (AHP281) following enzume mediated antigen retrieval using proteinase K

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  • IL-1 Alpha Antibody thumbnail image 1
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  • Rabbit anti Human Interleukin-1 Alpha
  • Rabbit anti Human Interleukin-1 Alpha:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP281GE, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP281G
    AHP281BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP281B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Interleukin-1 alpha antibody recognizes human IL-1 alpha (Interleukin-1 alpha), an 18kDa multi-functional pro-inflammatory cytokine which belongs to the IL-1 family of proteins, and is produced by a variety of cells including activated macrophages, monocytes and keratinocytes.

      IL-1 alpha and IL-1 beta have been identified as two distinct IL-1 proteins which bind to the same type 1 Interleukin 1 receptor (IL1R1). Biological properties of IL-1 alpha include stimulation of thymocyte proliferation by induction of IL-2 release, B-cell proliferation and maturation, and fibroblast growth factor activity.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human IL-1 alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
      Recombinant human IL-1alpha. (PHP040).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of cell division
      anti-apoptosis
      cytokine-mediated signaling pathway
      cell proliferation
      positive regulation of angiogenesis
      interleukin-1 receptor binding
      positive regulation of mitosis
      copper ion binding
      apoptosis
      cellular response to heat
      positive regulation vascular endothelial growth factor production
      cytokine activity
      extracellular space
      immune response
      response to copper ion
      negative regulation of cell proliferation
      fever generation
      positive regulation of cytokine secretion
      positive regulation of interleukin-2 biosynthetic process
      cytosol
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Functional Assays1.02.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP281B as the detection reagent.
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AHP281G as the capture reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Alpha Antibody Formats

    Formats Applications Sizes available
    IL-1 Alpha Antibody : Biotin E, WB 50 µg
    IL-1 Alpha Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

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