IKK Antibody (PrecisionAbTM Antibody)

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IKK Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with IKK antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to IKK (molecular weight 85 & 87 kDa).

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  • IKK Antibody with Control Lysate
  • Mouse anti IKK
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    IKK antibody recognizes inhibitor of nuclear factor kappa-B kinase subunit alpha, also known as I-kappa-B kinase 1, IKK-a kinase, IkB kinase alpha subunit or Nuclear factor NFkappaB inhibitor kinase alpha.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00667WBdatasheet pdfdatasheet pdf100 µl
      VMA00667
      VMA00667KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00667KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human IKK antibody recognizes inhibitor of nuclear factor (NF) kappa-B kinase subunit alpha, also known as I-kappa-B kinase 1, IKK-a kinase, IkB kinase alpha subunit or nuclear factor NFkappaB inhibitor kinase alpha.

        CHUK encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor (provided by RefSeq, Jul 2008).

        Mouse anti Human IKK antibody detects bands of 85 and 87 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti IKK antibody detects bands of approximately 85 and 87 kDa in K562 cell lysates
        Mouse anti IKK antibody detects bands of approximately 85 and 87 kDa in K562 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        Full length recombinant IKK
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        identical protein binding
        positive regulation of NF-kappaB transcription factor activity
        I-kappaB phosphorylation
        toll-like receptor 3 signaling pathway
        Toll signaling pathway
        innate immune response
        IkappaB kinase activity
        positive regulation of I-kappaB kinase/NF-kappaB cascade
        T cell receptor signaling pathway
        nerve growth factor receptor signaling pathway
        toll-like receptor 2 signaling pathway
        nucleus
        toll-like receptor 1 signaling pathway
        internal side of plasma membrane
        MyD88-dependent toll-like receptor signaling pathway
        ATP binding
        MyD88-independent toll-like receptor signaling pathway
        toll-like receptor 4 signaling pathway
        CD40 receptor complex
        phosphatidylinositol-mediated signaling
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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