IgG4 gallery image 1

Published customer image:
Native Human IgG4 used as a standard for the development of a human IgG4 ELISA.
Image caption:
Anti-cE5 IgG1 and IgG4 responses.
(A) Box plots of anti-cE5 IgG1 and IgG4 levels among the 207 individuals of the Aug 1994 survey. Boxes, whiskers, dots and P value as in Figure 1. IgG1 and IgG4 levels are expressed in ng/ml. (B) Scatter plot reporting the IgG1 (red) and IgG4 (black) antibody response to cE5 as function of age (n?=?207). The best-fit lines (solid) and 95% CI (broken lines) are shown. Note that three data points are outside the axis limits.

From: Rizzo C, Lombardo F, Ronca R, Mangano V, Sirima SB, Nèbiè I, Fiorentino G, Modiano D, Arcà B.
Differential antibody response to the Anopheles gambiae gSG6 and cE5 salivary proteins in individuals naturally exposed to bites of malaria vectors.
Parasit Vectors. 2014 Nov 28;7:549.

  • IgG4 thumbnail image 1
  • Native Human IgG4
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  • Product Type
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5254-3004Edatasheet pdfdatasheet pdf1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Native Human IgG4 is a purified preparation of human IgG4 from myeloma plasma. A single arc was observed in immunoelectrophoresis against antisera to whole human serum and human kappa light chain.
    • Intended Use
    • Target Species
    • Product Form
      Purified immunoglobulin from human myeloma serum - liquid
    • Reconstitution
    • Preparation
      Affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
      SDS PAGE: >95%
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG4 Formats

    Formats Applications Sizes available
    IgG4 : Purified E 1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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