Human IgG3 | AbD00264_hIgG3

Human IgG3 | AbD00264_hIgG3 gallery image 1

ELISA analysis of recombinant Human IgG3 lambda

Recombinant Human IgG3 lambda (HCA178) used as an antigen in an indirect ELISA followed by Mouse anti Human IgG (5172-6004) and HRP conjugated Rabbit F(ab')2 anti Mouse IgG (STAR13B)

Human IgG3 | AbD00264_hIgG3 gallery image 2

SDS-PAGE analysis of recombinant Human IgG3 lambda

Recombinant Human IgG3 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image

Human IgG3 | AbD00264_hIgG3 gallery image 3

Size exclusion chromatography of recombinant Human IgG3 lambda

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG3 lambda. The larger peak corresponds to monomeric, fully assembled AbD264_hIgG3. The smaller shoulder most likely contains dimers and aggregates

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  • Human IgG3 | AbD00264_hIgG3 thumbnail image 3
  • Recombinant Human IgG3 Lambda
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  • Product Type
    Recombinant Protein
  • Clone
  • Isotype
    IgG3 Lambda
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA178Edatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Recombinant Human IgG3 lambda, clone AbD264_hIgG3 is a recombinant Human IgG3 antibody containing a lambda light chain. The heavy chain belongs to the G3m(b1), previously designated G3m5, allotype.

      Clone AbD264_hIgG3 is specific for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Product Form
      Human IgG3 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein (GFP).
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human IgG3 Formats

    Formats Clone Applications Sizes available
    Human IgG3 : Purified AbD00264_hIgG3 E 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC*, E
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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