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IgG Antibody | A57H

IgG Antibody | A57H gallery image 1

Published customer image:
Mouse anti Human IgG (CH3 domain) antibody, clone A57H used as a coating antibody in a capture ELISA for vaccibodies contaiing the IgG (CH3 domain).
Image caption:
Characterization of fusion vaccines used in this study. (A) Schematic structure of a homodimeric vaccine fusion protein (Vaccibody, VB). Targeting, dimerization and antigenic units are indicated, as are the different moieties expressed in the various units. MHC class II targeted (I-Ed-specific) and non-targeted (NIP-specific) constructs are denoted (FvI-EFvPn)2< and (FvNIPFvPn)2, respectively, whereas LD78β VB are denoted (LD78βFvPn)2. where Pn indicates patient number. (B) Detection of VB proteins in supernatants of transfected HEK 293E cells in an ELISA specific for hγCH3. (C) Binding of MHC class II-targeted VB to I-Ed+ A20 cells. (D) LD78β VB displays dose-dependent chemotactic activity on lymphocytic Esb/MP cells. Recombinant LD78β was used as positive control.

From: Ruffini PA, Os A, Dolcetti R, Tjønnfjord GE, Munthe LA, Bogen B.
Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
J Transl Med. 2014 Jul 25;12:207.

  • IgG Antibody | A57H thumbnail image 1
  • Mouse anti Human IgG (CH3 Domain)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA878GE, H, WBdatasheet pdfdatasheet pdf0.2 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human IgG (CH3 Domain) antibody, clone A57H reacts with the CH3 domain of human IgG
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Clone Applications Sizes available
    IgG Antibody : Purified A57H E, H, WB 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IgG Antibody | A57H thumbnail image 1
              • Application NameReference Images
                Functional Assays


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