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IgG Antibody
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Enlarge- Goat anti Human IgG (H/L):HRP
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Goat anti Human IgG antibody is specific for Human IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
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ReconstitutionReconstitute with 1ml deionised water. For extended storage aliquot and freeze.
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Preparation
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Antiserum PreparationGoat anti Human IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography with human IgG coupled to agarose beads followed by solid phase adsorbtion to remove unwanted cross reactivity.
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Preservative Stabilisers
0.01% Gentamicin sulphate 1% Bovine Serum Albumin -
ImmunogenHuman IgG, whole molecule.
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Purity
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Approx. Protein ConcentrationsIg concentration 2.0 mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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StoragePrior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life12 months from date of reconstitution.
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GO Termscomplement activation, classical pathwayextracellular regioninnate immune responseprotein bindingmembrane fractionimmune responseantigen binding
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA 1/25,000 1/100,000 Immunohistology - Frozen 1/1,000 1/5,000 Western Blotting 1/2,000 1/10,000 - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional IgG Antibody Formats
| Formats | Applications | Sizes available |
|---|---|---|
| IgG Antibody : HRP | C, E, WB | 2 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C, E, P, WB | BUF052A | |
| TMB Signal+ | BUF054A | 100 ml | E | BUF054A | |
| TMB Core | BUF056A | 100 ml | E | BUF056A | |
| TMB Core Prestained | BUF057A | 100 ml | E | BUF057A | |
| TMB Core+ | BUF062A | 100 ml | E | BUF062A | |
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C, E, P, WB | BUF052C | |
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C, E, P, WB | BUF052B |
Recommended Positive Controls
Histology Controls
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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