IGF1R Antibody (PrecisionAbTM Antibody)

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IGF1R Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with IGF1R antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 52 second exposure. Arrow points to IGF1R (molecular weight 95 kDa)

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  • IGF1R Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti IGF1R
  • IGF1R Antibody with Control Lysate
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00674WBdatasheet pdfdatasheet pdf100 µl
    VPA00674
    VPA00674KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00674KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Rabbit anti Human IGF1R antibody recognizes the insulin-like growth factor 1 receptor, also known as IGF-I receptor, soluble IGF1R variant 1, IGFR or soluble IGF1R variant 2.

      This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival (provided by RefSeq, Jul 2008).

      Rabbit anti Human IGF1R antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti IGF1R detects a band of approximately 95 kDa in Jurkat cell lysates
      Rabbit anti IGF1R detects a band of approximately 95 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Rabbit polyclonal antibody purified by affinity chromatography
      20μl Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
    • Immunogen
      Synthetic peptide encompassing part of the middle region of human IGF1R
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
    • Regulatory
      For research purposes only
    • GO Terms
      insulin receptor signaling pathway
      positive regulation of DNA replication
      identical protein binding
      insulin-like growth factor I binding
      phosphatidylinositol-mediated signaling
      protein tetramerization
      ATP binding
      insulin-like growth factor receptor activity
      metal ion binding
      positive regulation of cell migration
      protein autophosphorylation
      anti-apoptosis
      positive regulation of cell proliferation
      insulin binding
      phosphatidylinositol 3-kinase cascade
      insulin receptor binding
      microsome
      integral to plasma membrane
      insulin receptor substrate binding
      phosphatidylinositol 3-kinase binding
      immune response
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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