IGF-II Antibody

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IGF-II Antibody gallery image 1

Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human insulin-like growth factor II (AHP776)

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IGF-II Antibody gallery image 2

Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human insulin-like growth factor II (AHP776)

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IGF-II Antibody gallery image 3

Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human insulin-like growth factor II (AHP776)

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  • IGF-II Antibody thumbnail image 1
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  • Rabbit anti Human Insulin-Like Growth Factor II
  • Rabbit anti Human Insulin-Like Growth Factor II:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP776E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP776
    AHP776BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP776B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Insulin-Like Growth Factor II antibody recognizes human IGF-II (Insulin-like Growth Factor II), a 67 amino acid mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen.

      IGF-II is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulin-like growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGF-II is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (Singer et al. 2004).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
      Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 0.1 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of cell proliferation
      positive regulation of insulin receptor signaling pathway
      growth factor activity
      positive regulation of MAPKKK cascade
      insulin receptor binding
      skeletal system development
      positive regulation of protein kinase B signaling cascade
      extracellular space
      positive regulation of cell division
      glucose metabolic process
      insulin-like growth factor receptor binding
      hormone activity
      positive regulation of mitosis
      regulation of gene expression by genetic imprinting
      ossification
      regulation of gene-specific transcription
      insulin receptor signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays3.14.6ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human IGF-II antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IGF-II antibody (AHP776B) as the detection reagent and recombinant human IGF-II (PHP034A) as the standard.
    • ELISA
      This biotinylated human IGF-II antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IGF-II antibody (AHP776) as the capture reagent and recombinant human IGF-II (PHP034A) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IGF-II (PHP034A) as the positive control.
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IGF-II (PHP034A) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional IGF-II Antibody Formats

    Formats Applications Sizes available
    IGF-II Antibody : Purified E, FN, WB 0.1 mg
    IGF-II Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Insulin-Like Growth Factor II:BiotinAHP776B50 µgE, WB
          AHP776B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Insulin-Like Growth Factor IIAHP7760.1 mgE, FN, WB
          AHP776

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Insulin-Like Growth Factor IIPHP034A50 µgE, FN, WB
            PHP034A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Insulin-Like Growth Factor IIPHP034A50 µgE, FN, WB
            PHP034A

            Histology Controls

              References

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