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IgE gallery image 1

Sandwich ELISA analysis of human IgE using Mouse anti Human IgE (MCA2115) as a capture reagent and horseradish peroxidase conjugated Mouse anti Human IgE (MCA2216P) as a detection reagent with purified human IgE (PHP142) as antigen. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A serum sample at 1/10 (blue) and 1/5 (green) and a plasma sample at 1/10 (red) and1/5 (orange) is shown

  • IgE thumbnail image 1
  • Human IgE Lambda Myeloma
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP142Edatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Purified human IgE from a human myeloma secreting IgE with a λ light chain yields a single arc by immunoelectrophoresis against antisera to whole human serum, human IgE and human lambda light chain.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgE myeloma protein - liquid
    • Reconstitution
    • Preparation
      Prepared by ion exchange chromatography from serum.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Purity
      >95% by SDS PAGE
    • Approx. Protein Concentrations
      1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgE Formats

    Formats Applications Sizes available
    IgE : Purified E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IgE thumbnail image 1


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