IgE Antibody | 1A2

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Sandwich ELISA analysis of human IgE using Mouse anti Human IgE (MCA2115) as a capture reagent and horseradish peroxidase conjugated Mouse anti Human IgE (MCA2216P) as a detection reagent with purified human IgE (PHP142)as antigen. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A serum sample at 1/10 (blue) and 1/5 (green) and a plasma sample at 1/10 (red) and1/5 (orange) is shown

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  • Mouse anti Human IgE:HRP
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  • Product Type
    Monoclonal Antibody
  • Clone
    1A2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2116PEdatasheet pdfdatasheet pdf0.5 mg
    MCA2116P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human IgE antibody, clone 1A2 recognizes human IgE and no cross-reactivity to other human immunoglobulin classes or subclasses has been detected.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.01%Thiomersal
    • Immunogen
      Chimaeric human IgE.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      MCA2116P can be used in a sandwich ELISA as a detection antibody together with MCA2115 as the capture antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgE Antibody Formats

    Formats Clone Applications Sizes available
    IgE Antibody : HRP 1A2 E 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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