IgD Antibody | LE2

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IgD Antibody | LE2 gallery image 1

Purification of IgD. (a) Elution profile of the final Superdex S200 gel-filtration column. IgD was eluted as a single peak from which fractions 2–4 were concentrated for the scattering experiments. (b) SDS-PAGE analysis of IgD (lane 1) and molecular mass markers (lane 2) run under reducing conditions and stained with Coomassie brilliant blue. Molecular masses are shown in kDa. (c) Western blot analysis of IgD (lane 1) from reducing SDS-PAGE. Image from: Sun et al (2005). JMB 353: 155-73.
Western blot detection of IgD accomplished using Mouse anti Human IgD (Fc) (MCA644G)

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  • IgD Antibody | LE2 thumbnail image 1
  • Mouse anti Human IgD (Fc)
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  • Product Type
    Monoclonal Antibody
  • Clone
    LE2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA644GCdatasheet pdfdatasheet pdf0.2 mg
    MCA644G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human IgD (Fc) antibody, clone LE2 recognizes an epitope located in the Fc region the heavy chain of human immunoglobulin D (IgD). Human IgD existst in two forms, the major membrane bound form which is co-expressed with IgM on the surface of mature B-cells (Blattner et al. 1984) and a minor secreted form comprising about 0.25% total serum Ig (Rogentine et al. 1966). Membrane bound IgD bears predominantly the κ light chain whereas the secreted form has predominantly λ light chains (Preud'homme et al. 2000)
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Purified Human IgD (Fc).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1001/300

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgD Antibody Formats

    Formats Clone Applications Sizes available
    IgD Antibody : Purified LE2 C 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
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              References

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