IgA Antibody

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Biotinylated Goat anti Human IgA antibody used for the evaluation of human IgA responses to pneumococcal challange by ELISA with subsequent detection by alkaline phosphatase conjugated streptavidin (STAR6B) and p-nitrophenyl phosphate.
Image caption:
IgG and IgA responses to whole cell 23F or 6B pneumococci following 23F or 6B challenge, respectively. ELISAs were performed using 23F or 6B pneumococci as targets to measure specific IgG (A, C and E) and IgA (B, D and F) titers in serum (A and B), NW (C and D) and BAL (E and F). Values shown are the mean antibody titers (of triplicates), pre and post 23F (n?=?7) or 6B (n?=?8) challenge (x-axis). *represents statistical significance between pre- and post-inoculation antibody titers.

From: Wright AKA, Ferreira DM, Gritzfeld JF, Wright AD, Armitage K, Jambo KC, et al. (2012)
Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog 8(4): e1002622.

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  • IgA Antibody thumbnail image 1
  • Goat anti Human IgA:Alk. Phos.
  • Goat anti Human IgA:Biotin
  • Goat anti Human IgA:RPE
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    205009Fdatasheet pdfdatasheet pdf0.5 mg
    205009
    205008Edatasheet pdfdatasheet pdf1 mg
    205008
    205004Edatasheet pdfdatasheet pdf1 ml
    205004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human IgA antibody recognizes the heavy chain of human IgA as demonstrated by ELISA and Flow cytometry. IgA is the major catagory of antibody present in mucosal secretions and acts as a first line of defense against inhaled or ingested pathogens at the mucosal interface. IgA is also present in serum where it acts to eliminate pathogens via interaction with FcαR, also known as CD89. (Woof et al. 2004).
      Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Ig Fraction conjugated to Biotin - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography on pooled human IgA
    • Antiserum Preparation
      Antisera to human IgA were raised by repeated immunisations of goats with highly purified antigen.
    • Preservative Stabilisers
      0.1%Sodium Azide
      50%Glycerol
      0.1% Sodium Azide (NaN3)
      0.1% Sodium azide
      Stabilising agent
    • Immunogen
      Human IgA.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5mg/ml
      0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      +4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
    • Shelf Life
      Please see label for expiry date.
      12 months from date of despatch.
      18 months from the date of despatch.
    • GO Terms
      membrane fraction
      antigen binding
      protein-chromophore linkage
      protein binding
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : RPE F 0.5 mg
    IgA Antibody : Biotin E 1 mg
    IgA Antibody : Alk. Phos. E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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