IFN Gamma antibody | B27

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1		Neat	1/10

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

References for IFN Gamma antibody

1. Paliard, X. et al. (1988) Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.
   J Immunol. 141 (3): 849-55.
2. Favre, C. et al. (1989) Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.
   Mol Immunol. 26 (1): 17-25.
3. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.
4. Wakkach, A. et al. (2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.
   J Immunol. 167: 3107-13.
5. Gomes, J.A. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.
   Infect Immun. 71 (3): 1185-93.
6. Lai, X. et al. (2003) Immune biology of macaque lymphocyte populations during mycobacterial infection.
   Clin Exp Immunol. 133: 182-92.
7. Dieli-Crimi, R. et al. (2018) Th1-skewed profile and excessive production of proinflammatory cytokines in a NFKB1-deficient patient with CVID and severe gastrointestinal manifestations.
   Clin Immunol. 195: 49-58.

ELISpot

Flow Cytometry

Synonyms

Interferon Gamma

RRID

AB_566998

UniProt

P01579

Entrez Gene

IFNG

GO Terms

GO:0000060 protein import into nucleus, translocation

GO:0002053 positive regulation of mesenchymal cell proliferation

GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis

GO:0009615 response to virus

GO:0005125 cytokine activity

GO:0005133 interferon-gamma receptor binding

GO:0005615 extracellular space

GO:0006955 immune response

GO:0007050 cell cycle arrest

View More GO Terms

GO:0050796 regulation of insulin secretion

GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter

GO:0032735 positive regulation of interleukin-12 production

GO:0032747 positive regulation of interleukin-23 production

GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein

GO:0034393 positive regulation of smooth muscle cell apoptosis

GO:0042508 tyrosine phosphorylation of Stat1 protein

GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein

GO:0045429 positive regulation of nitric oxide biosynthetic process

GO:0045672 positive regulation of osteoclast differentiation

GO:0048662 negative regulation of smooth muscle cell proliferation

GO:0051044 positive regulation of membrane protein ectodomain proteolysis

GO:0051712 positive regulation of killing of cells of other organism

GO:0060333 interferon-gamma-mediated signaling pathway

GO:0060334 regulation of interferon-gamma-mediated signaling pathway

GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity

GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process

GO:0060557 positive regulation of vitamin D biosynthetic process

GO:0060559 positive regulation of calcidiol 1-monooxygenase activity

GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation

MCA1554F

153117

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