IFN Gamma Antibody | B27

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IFN Gamma Antibody | B27 gallery image 1

Staining of activated human peripheral blood lymphocytes with Mouse anti Human Interferon Gamma: FITC (MCA1554FITC)

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  • IFN Gamma Antibody | B27 thumbnail image 1
  • Mouse anti Human Interferon Gamma:FITC
  • Mouse anti Human Interferon Gamma:RPE
  • Mouse anti Human Interferon Gamma:APC
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  • Product Type
    Monoclonal Antibody
  • Clone
    B27
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1554FF *datasheet pdfdatasheet pdf0.1 mg
    MCA1554F
    MCA1554PEF *datasheet pdfdatasheet pdf100 Tests
    MCA1554PE
    MCA1554APCF *datasheet pdfdatasheet pdf100 Tests
    MCA1554APC
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Interferon gamma antibody, clone B27 recognizes human interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.

      Mouse anti Human Interferon gamma antibody, clone B27 is reported to neutralize the antiviral activity of human IFN gamma (Favre et al. 1989).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
    • Reconstitution
      Reconstitute with 1 ml distilled water
      Reconstitute with 1.0ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Recombinant human IFN gamma.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
      positive regulation of calcidiol 1-monooxygenase activity
      positive regulation of mesenchymal cell proliferation
      positive regulation of nitric oxide biosynthetic process
      interferon-gamma-mediated signaling pathway
      positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      extracellular space
      regulation of insulin secretion
      immune response
      cell cycle arrest
      positive regulation of fructose 1,6-bisphosphate metabolic process
      positive regulation of interleukin-12 production
      negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
      negative regulation of smooth muscle cell proliferation
      positive regulation of killing of cells of other organism
      positive regulation of peptidyl-serine phosphorylation of STAT protein
      positive regulation of smooth muscle cell apoptosis
      cytokine activity
      tyrosine phosphorylation of Stat1 protein
      protein import into nucleus, translocation
      positive regulation of interleukin-23 production
      response to virus
      regulation of interferon-gamma-mediated signaling pathway
      interferon-gamma receptor binding
      negative regulation of metanephric nephron tubule epithelial cell differentiation
      positive regulation of osteoclast differentiation
      positive regulation of membrane protein ectodomain proteolysis
      positive regulation of vitamin D biosynthetic process
      positive regulation of tyrosine phosphorylation of Stat1 protein
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : FITC B27 F * 0.1 mg
    IFN Gamma Antibody : RPE B27 F * 100 Tests
    IFN Gamma Antibody : APC B27 F * 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:APCMCA928APC100 TestsF
        MCA928APC

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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