Recombinant human interferon beta 1b produced in E. coli is a single non-glycosylated variant form of the human interferon beta-1b polypeptide chain. The interferon gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine at residue 17.
Purified Recombinant protein - lyophilised.
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant Interferon beta 1b expressed in E. coli.
The Specific activity determined using a viral resistance assay is of 32 x 106 IU/mg. The viral resistance assays used are the human WISH cell line and VSV, or the monkey VERO cell line and EMCV.
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
18.5 kDa (165 amino acid residues)
Reagents In The Kit
Preparing The Antibody
Lyophilised from a solution containing human albumin and dextrose.
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.