Recombinant human interferon beta 1a is a CHO cell derived recombinant protein corresponding to single, glycosylated polypeptide chain containing 166 amino acid. Interferon beta 1a is a 22 kDa cytokine and member of the type 1 interferon family.
Interferons exhibit anti-viral and anti proliferative effects and also influence the immune response. Effects on the immune system mediated by interferons may be mediated by increased expression of major histocompatability complex (MHC) antigens. Interferon beta a has been shown to exhibit therapeutic efficacy against viral infections, different types of cancer and multiple sclerosis (Borden et al. 2007).
Recombinant protein - lyophilised
Reconstitute with 50ul of distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
0.34M Mannitol 0.9% human serum albumin
270 x 106 IU/mg, as determined in a viral resistance assay
>98.0% as determined by HPLC and SDS-PAGE.
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Reagents In The Kit
Preparing The Antibody
0.01M Sodium Acetate
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.