Recombinant human lFN-α; A expressed in Escherichia coli.
N.B. Antibody reactivity and working conditions may vary between species.
Purified recombinant protein - liquid
Prepared by a combination of ion exchange, hydrophobic interactions and size exclusion chromatography.
Bovine Serum Albumin
8.5 x 107 units/ml
Activity is determined via cyopathic inhibition assay on MDBK cells.
>95% by SDS PAGE
Approx. Protein Concentrations
Protein Molecular Weight
Reagents In The Kit
Preparing The Antibody
< 1 EU/ug
Phosphate buffered saline
Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
2. Mathieu, M.G. et al. (2014) The helicase HAGE prevents interferon-a-induced PML expression in ABCB5+ malignant melanoma-initiating cells by promoting the expression of SOCS1.Cell Death Dis. 5:e1061.