I-FABP Antibody (PrecisionAbTM Antibody) | AB15/4F8

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I-FABP Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB15/4F8 gallery image 1

Western blot analysis of tissue lysate probed with I-FABP antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to I-FABP (molecular weight 15 kDa)

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  • I-FABP Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB15/4F8 thumbnail image 1
  • Mouse anti I-FABP
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2a
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00552WBdatasheet pdfdatasheet pdf100 µl
    VMA00552
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Mouse anti Human I-FABP antibody recognizes I-FABP, also known as FABP2 and fatty acid-binding protein, intestinal.

      The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance (provided by RefSeq, Jul 2008).

      Mouse anti Human I-FABP antibody detects a band of 15 kDa. The antibody has been extensively validated for western blotting using human intestine tissue lysate.
    • Target Species
      Human
    • Western Blotting
      Anti I-FABP detects a band of approximately 15 kDa in human intestinal tissue lysates
    • Product Form
      Purified IgG - liquid
    • Preparation
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
    • Buffer Solution
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli-derived recombinant human I-FABP (amino acids 1 - 132)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
    • Lysate Reconstitution
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      fatty acid binding
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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