Hsc70 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-hsc70-antibody-vma00600.html?RenderPageSpecific=1
Hsc70 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with Hsc70 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to Hsc70 (molecular weight 73 kDa).

Enlarge
  • Hsc70 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Hsc70 Antibody with Control Lysate
  • Human anti Hsc70
(Rated 0.0 out of 5 based on 0 customer reviews)
    Hsc70 antibody recognizes heat shock protein family A (Hsp70) member 8 also known as LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70 or heat shock cognate protein 54.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00600WBdatasheet pdfdatasheet pdf100 µl
      VMA00600
      VMA00600KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00600KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human Hsc70 antibody recognizes the heat shock protein family A (Hsp70) member 8 also known as LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70 or heat shock cognate protein 54.

        This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Aug 2011).

        Human anti Human Hsc70 antibody detects a band of 73 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Human anti Hsc70 antibody detects a band of approximately 73 kDa in K562 cell lysates
        Human anti Hsc70 antibody detects a band of approximately 73 kDa in K562 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
        Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E. coli-derived recombinant human Hsc70 protein (aa 1-646)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        protein binding
        clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
        protein folding
        plasma membrane
        ribonucleoprotein complex
        interspecies interaction between organisms
        mRNA metabolic process
        ATP binding
        response to unfolded protein
        post-Golgi vesicle-mediated transport
        neurotransmitter secretion
        cell surface
        ATPase activity, coupled
        cytosol
        cellular membrane organization
        melanosome
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "Hsc70" specificity.