Hsc70 Antibody

Hsc70 Antibody gallery image 1

Western blot analysis of Hsc70 expression in A431 cell line lysate using Goat anti Human Hsc70 antibody (AHP1571) followed by HRP conjugated anti goat IgG. Detection by chemiluminescence

Hsc70 Antibody gallery image 2

Western blotting analysis of HepG2 (A) and NIH3T3 (B) cell lysate (35μg protein in RIPA buffer) probed with Goat anti Human Hsc70 antibody (AHP1571) used at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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  • Goat anti Human Hsc70
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1571E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human Hsc70 antibody recognizes human Heat shock cognate 71 kDa protein, also known as Heat shock 70 kDa protein 8 or Lipopolysaccharide-associated protein 1. Hsc70 is a 646 amino acid ubiquitously expressed cytoplasmic heat-shock cognate protein and member of the heat shock protein 70 family, encoded for by the HSPA8 gene. Goat anti Human Hsc70 antibody recognizes epitopes in the region between amino acids 555 and 570 of Hsc70 isoform 1, missing in isoform 2.

      Heat shock proteins are a group of constitutively expressed or heat/stress-inducible proteins which act as molecular chaperones and play an essential role in protein folding/unfolding and transport of proteins across membranes. Hsc70 facilitates the correct folding of newly-synthesized polypeptides (Flynn et al. 1989) and also participates in the ATPase disassociation of clathrin-coated vesicles during endocytosis, in conjunction with the co-chaperone auxilin (Jiang et al. 1997).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      This product detects a band of approximately 75kDa in A431 cell lysates.
    • Instructions For Use

    Additional Hsc70 Antibody Formats

    Formats Applications Sizes available
    Hsc70 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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