hnRNP A Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-hnrnp-a-antibody-vma00634.html?RenderPageSpecific=1
hnRNP A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with hnRNP A antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to hnRNP A (molecular weight 34 kDa).

Enlarge
  • hnRNP A Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • hnRNP A Antibody with Control Lysate
  • Human anti hnRNP A
(Rated 0.0 out of 5 based on 0 customer reviews)
    hnRNP A antibody recognizes heterogeneous nuclear ribonucleoprotein A1, also known as putative heterogeneous nuclear ribonucleoprotein A1-like 3, helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1B protein or single-strand DNA-binding protein UP1.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00634WBdatasheet pdfdatasheet pdf100 µl
      VMA00634
      VMA00634KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00634KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human hnRNP A antibody recognizes heterogeneous nuclear ribonucleoprotein A1, also known as putative heterogeneous nuclear ribonucleoprotein A1-like 3, helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1B protein, heterogeneous nuclear ribonucleoprotein B2 protein, heterogeneous nuclear ribonucleoprotein core protein A1, hnRNP A1-like 3, hnRNP core protein A1, hnRNP core protein A1-like 3, nuclear ribonucleoprotein particle A1 protein, single-strand DNA-binding protein UP1, single-strand RNA-binding protein.

        HNRNPA1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by HNRNPA1 has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for HNRNPA1 but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites (provided by RefSeq, Jul 2008).

        Human anti Human hnRNP A antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Human anti hnRNP A antibody detects a band of approximately 34 kDa in Jurkat cell lysates
        Human anti hnRNP A antibody detects a band of approximately 34 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
        Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E. coli-derived recombinant human hnRNP A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        spliceosomal complex
        nucleoplasm
        interspecies interaction between organisms
        nucleotide binding
        nucleolus
        cytoplasm
        nuclear import
        heterogeneous nuclear ribonucleoprotein complex
        single-stranded RNA binding
        mRNA transport
        RNA export from nucleus
        single-stranded DNA binding
        protein binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "hnRNP A" specificity.