HLA-DQA2 Antibody (PrecisionAbTM Antibody)

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HLA-DQA2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with HLA-DQA2 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 2 second exposure. Arrow points to HLA-DQA2 (molecular weight 28 kDa)

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  • Rabbit anti HLA-DQA2
  • HLA-DQA2 Antibody with Control Lysate
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    Rabbit anti-human HLA-DQA2 antibody recognizes the HLA class II histocompatibility antigen, DQ alpha 2 chain, also known as DX alpha chain, HLA class II histocompatibility antigen or MHC class II DQA2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00443WBdatasheet pdfdatasheet pdf100 µl
      VPA00443
      VPA00443KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00443KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human HLA-DQA2 antibody recognizes the HLA class II histocompatibility antigen DQ alpha 2 chain, also known as DX alpha chain, HLA class II histocompatibility antigen, DQ (6) alpha chain or MHC class II DQA2.

        HLA-DQA2 belongs to the HLA class II alpha chain family. The HLA-DQA2 gene encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B-cells, dendritic cells, macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells (provided by RefSeq, Jun 2010).

        Rabbit anti Human HLA-DQA2 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti HLA-DQA2 detects a band of approximately 28 kDa in Raji cell lysates
        Anti HLA-DQA2 detects a band of approximately 28 kDa in Raji cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography
        20μl Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide corresponding to amino acid 39-66 of human HLA-DQA2
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Raji lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        Golgi apparatus
        T cell receptor signaling pathway
        integral to plasma membrane
        endosome membrane
        MHC class II protein complex
        interferon-gamma-mediated signaling pathway
        T cell costimulation
        immune response
        endoplasmic reticulum membrane
        MHC class II receptor activity
        lysosomal membrane
        antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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