Histone H2AX Antibody

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Detection of human and mouse histone H2AX in nuclear extracts from human 293T, human G-361 and wild type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts by Western blotting using Rabbit anti Human H2AX (AHP838)

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  • Rabbit anti Human Histone H2AX
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP838IP, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Rabbit anti Human Histone 2AX antibody recognizes human histone 2AX (H2AX), a 143 amino acid, ~15kDa member of the histone H2A family differing from other members in having a unique carboxy-terminal region containing a conserved serine, four bases from the carboxy terminus (Redon 2002) which becomes rapidly phosphorylated following DNA double stranded breaks (Rogakou et al. 1998).

      H2AX is involved in DNA repair and the maintenance of genomic stability, although its precise role is still to be defined. H2AX is phosphorylated soon after the appearance of double-stranded breaks, and has been implicated both in homologous recombination and non-homologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins (Scully and Xie 2013). Phoshorylation of H2AX is linked to DNA fragmentation during apoptosis (Rogaku et al. 2000).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation5ug/mg protein20ug/mg protein
      Western Blotting1/50001/15000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP838 detects a band of approximately 15kDa in nuclear extracts from human 293 T cells.
    • Instructions For Use

    Additional Histone H2AX Antibody Formats

    Formats Applications Sizes available
    Histone H2AX Antibody : Purified IP, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

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          Recommended Positive Controls

            Histology Controls

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