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High Mobility Group Protein B2 antibody

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Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2
Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of High Mobility Group Protein B2 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Rabbit anti High Mobility Group Protein B2 antibody (VPA00494) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 29 second and (B) 30 second exposure.
Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Normalized signal intensity of High Mobility Group Protein B2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti High Mobility Group Protein B2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
High Mobility Group Protein B2

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Product Code Applications Pack Size List Price Your Price Qty
VPA00494
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Rabbit anti Human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2, high mobility group protein 2, high-mobility group (non-histone chromosomal) protein 2 or high-mobility group box 2.

HMG-2 gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro HMG-2 is able to efficiently bend DNA and form DNA circles (Paull et al. 1993) suggesting a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. HMG-B2 is involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination (Nagaki et al. 1998) .

Rabbit anti Human high mobility group protein B2 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti high mobility group protein B2 detects a band of approximately 28 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the C terminal region of human high mobility group protein B2
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml loader
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Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P26583
Entrez Gene
HMGB2
GO Terms
GO:0000793 condensed chromosome
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006334 nucleosome assembly
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006265 DNA topological change
GO:0006288 base-excision repair, DNA ligation
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0008301 DNA bending activity
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016565 general transcriptional repressor activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032075 positive regulation of nuclease activity
GO:0033151 V(D)J recombination
GO:0042056 chemoattractant activity
GO:0043234 protein complex
GO:0043388 positive regulation of DNA binding
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045654 positive regulation of megakaryocyte differentiation
GO:0048471 perinuclear region of cytoplasm
GO:0050786 RAGE receptor binding
GO:0060326 cell chemotaxis
GO:0071222 cellular response to lipopolysaccharide

VPA00494

160712

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