High Mobility Group Protein B2 Antibody (PrecisionAbTM Antibody)

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High Mobility Group Protein B2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with high mobility group protein B2 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 31 second exposure. Arrow points to high mobility group protein B2 (molecular weight 28 kDa)

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  • High Mobility Group Protein B2 Antibody with Control Lysate
  • Rabbit anti High Mobility Group Protein B2
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    Rabbit anti-human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2 or high-mobility group box 2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00494WBdatasheet pdfdatasheet pdf100 µl
      VPA00494
      VPA00494KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00494KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2, high mobility group protein 2, high-mobility group (nonhistone chromosomal) protein 2 or high-mobility group box 2.

        HMGB2 gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles (Paull et al. 1993). These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination (Nagaki et al. 1998) (provided by RefSeq, Jul 2008).

        Rabbit anti Human high mobility group protein B2 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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