HIF1 Alpha Antibody | ESEE122

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HIF1 Alpha Antibody | ESEE122 gallery image 1

Paraffin embedded human tonsil stained with Mouse anti Human Hif1α antibody, clone ESEE122 following antigen retrieval with EDTA. Visualization was achieved using the Histar detection system (STAR3000A)

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  • HIF1 Alpha Antibody | ESEE122 thumbnail image 1
  • Mouse anti Human HIF1 Alpha
  • Mouse anti Human HIF1 Alpha
  • Mouse anti Human HIF1 Alpha:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ESEE122
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5695GAE, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5695GA
    MCA5695BE, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5695B
    MCA5695E, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA5695
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human HIF1 alpha antibody, clone ESEE122 recognizes human HIF1α, also known as Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1 or PAS domain-containing protein 8. HIF1α is a widely expressed bHLH and PAS domain containing transcription factor, a critical regulatory protein in the host response to hypoxia.

      HIF1α interacts with the HIF1β/ARNT subunit to form the highly-conserved heterodimeric HIF-1 transcriptional complex. During hypoxic conditions, HIF1α translocated from a normoxic cytoplasmic location to the nucleus and activates transcription of several genes including erythropoietin, VEGF and glucose transporters, encoding proteins involved in oxygen delivery and metabolic adaptation to hypoxia. Under normal oxygen conditions, HIF1α is targeted by HIF prolyl-hydroxylase, followed by rapid proteolytic degradation. Overexpression of HIF1α occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Semenza 2003).

      Mouse anti Human HIF1 alpha antibody, clone ESEE122 has been shown to be suitable for the staining of snap-frozen cell pellets (Talks. et al. 2000).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Immunohistology - Paraffin(1)1/1001/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      EDTA pH9.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Immunohistology - Paraffin(1)1/1001/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      EDTA pH9.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      Special conditions apply - see [*]

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
      *This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase, or antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH9.0 is recommended for this purpose.
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA5695 detects a band of approximately 135kDa in 0.1% hypoxia HeLa cell lysates(1).
    • Western Blotting
      MCA5695GA detects a band of approximately 135kDa in 0.1% hypoxia HeLa cell lysates(1).
    • Western Blotting
      MCA5695B detects a band of approximately 135kDa in 0.1% hypoxia HeLa cell lysates(1).
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional HIF1 Alpha Antibody Formats

    Formats Clone Applications Sizes available
    HIF1 Alpha Antibody : Biotin ESEE122 E, P *, WB 0.1 mg
    HIF1 Alpha Antibody : Purified ESEE122 E, P *, WB 0.1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              Human tonsil
              Human tonsil

              References

              Further Reading

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