Hexokinase 1 Antibody | 4D7

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Hexokinase 1 Antibody | 4D7 gallery image 1

Immunofluorescent staininf of HeLa Cells with Mouse anti Human hexokinase 1 (MCA2698), green. Counterstained nuclei with Hoechst 33342, blue

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Hexokinase 1 Antibody | 4D7 gallery image 2

Western blotting analysis of hexokinase specificity using Mouse anti Human hexokinase 1, clone 4D7 (MCA2698)
A: hexokinase specificity against recombinant hexokinase proteins
B: hexokinase expression in human cell lines and mouse brain

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  • Hexokinase 1 Antibody | 4D7 thumbnail image 1
  • Hexokinase 1 Antibody | 4D7 thumbnail image 2
  • Mouse anti Human Hexokinase 1
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  • Product Type
    Monoclonal Antibody
  • Clone
    4D7
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2698E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2698
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human hexokinase 1, clone 4D7 recogniszs human hexokinase 1, a 102kDa major allosteric enzyme of the glycolytic pathway, which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).

      Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type 1 to 4), expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia (Newman et al. 1980).

      Mouse anti hexokinase 1, clone 4D7 recognizes an epitope common to all isoforms of human hexokinase.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Recombinant human hexokinase 1 (amino acids 1-917) purified from Escherichia coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/10001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2698 detects a band of approximately 102kDa in HepG2 cell lysates.
    • Instructions For Use

    Additional Hexokinase 1 Antibody Formats

    Formats Clone Applications Sizes available
    Hexokinase 1 Antibody : Purified 4D7 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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