Hexokinase 1 & 2 Antibody | 1A7

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Hexokinase 1 & 2 Antibody | 1A7 gallery image 1

Western blotting analysis of hexokinase specificity using Mouse anti Human hexokinase 1&2, clone 1A7 (MCA4625GA)
A: hexokinase specificity against recombinant hexokinase proteins
B: hexokinase expression in Jurkat cell lysate

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  • Hexokinase 1 & 2 Antibody | 1A7 thumbnail image 1
  • Mouse anti Human Hexokinase 1 & 2
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  • Product Type
    Monoclonal Antibody
  • Clone
    1A7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4625GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4625GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Hexokinase 1&2, clone 1A7 recognizes human hexokinase 1 (HK1) and hexokinase 2 (HK2), 102kDa allosteric enzymes of the glycolytic pathway, which catalyse the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).

      Hexokinase 1 is predominantly expressed in the brain while Hexokinase 2 is predominantly expressed in skeletal muscle. Both are members of four distinct mammalian hexokinase isozymes (hexokinase type I to IV), which localize to the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P (Wolf et al. 2011)
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/10001/3000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4625GA detects a band of approximately 102 kDa in Jurkat cell lysates.
    • Instructions For Use

    Additional Hexokinase 1 & 2 Antibody Formats

    Formats Clone Applications Sizes available
    Hexokinase 1 & 2 Antibody : Purified 1A7 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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