HEREMANS-SCHMID Glycoprotein Antibody (PrecisionAbTM Antibody)

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HEREMANS-SCHMID Glycoprotein Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell and human plasma lysates probed with Heremans Schmid glycoprotein antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to Heremans Schmid glycoprotein (molecular weight 58 kDa)

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  • HEREMANS-SCHMID Glycoprotein Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • HEREMANS-SCHMID Glycoprotein Antibody with Control Lysate
  • Goat anti HEREMANS-SCHMID Glycoprotein
  • HEREMANS-SCHMID Glycoprotein Antibody with Control Lysate
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    Goat anti human Heremans-Schmid glycoprotein antibody recognizes alpha-2 Heremans-Schmid glycoprotein, also known as fetuin A. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00153WBdatasheet pdfdatasheet pdf100 µl
      VPA00153
      VPA00153KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00153K
      VPA00153KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00153KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human Heremans-Schmid glycoprotein antibody recognizes Heremans-Schmid glycoprotein also known as Alpha-2-HS-glycoprotein , Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A and it is cleaved into the following 2 chains: Alpha-2-HS-glycoprotein chain A and Alpha-2-HS-glycoprotein chain B.

        Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure (provided by RefSeq, Jul 2008).

        Goat anti Human Heremans-Schmid glycoprotein antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti Heremans-Schmid glycoprotein detects a band of approximately 58 kDa in C6 cell lysates.
        Anti Heremans-Schmid glycoprotein detects a band of approximately 58 kDa in C6 cell lysates.
        Anti Heremans-Schmid glycoprotein detects a band of approximately 58 kDa in C6 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody prepared by ion exchange chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody prepared by ion exchange chromatography
        Goat polyclonal antibody prepared by ion exchange chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody prepared by ion exchange chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody prepared by ion exchange chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        Pack Size: 10 Westerns0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        Pack Size: 2 Westerns0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% BSA
        Pack Size: 10 Westerns0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        Pack Size: 2 Westerns0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        Recombinant human Heremans-Schmid glycoprotein processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg C6 lysate lyophilized in RIPA buffer.
        400μg C6 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        acute-phase response
        extracellular space
        regulation of inflammatory response
        negative regulation of bone mineralization
        protein binding
        kinase inhibitor activity
        cysteine-type endopeptidase inhibitor activity
        negative regulation of insulin receptor signaling pathway
        positive regulation of phagocytosis
        pinocytosis
        skeletal system development
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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