Heparanase-1 Antibody | HP3/17

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  • Mouse anti Human Heparanase-1
  • Mouse anti Human Heparanase-1
  • Mouse anti Human Heparanase-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HP3/17
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1975GP, WBdatasheet pdfdatasheet pdf0.1 mg
    OBT1975G
    OBT1975XP, WBdatasheet pdfdatasheet pdf0.15 mg
    OBT1975X
    OBT1975P, WBdatasheet pdfdatasheet pdf50 µg
    OBT1975
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Heparanase-1 antibody, clone HP3/17 recognizes heparanase-1, also know as HPA1, an endo-beta-D-glucuronidase that degrades heparan sulfate proteoglycans (HSPGs) in the extracellular matrix through cleavage of the heparan sulfate side chains.

      Heparanase-1 is a heterodimer that is produced as a 65 kDa latent precursor, consisting of an 8 kDa subunit, a 50 kDa subunit and a linker peptide. Activation of Heparanase-1 occurs through cleavage of the linker peptide, releasing the 8 kDa subunit from the active 50 Da subunit.

      Mouse anti Heparanase-1 has been reported to recognize heparanase-1 in xenopus by western blotting (Bertolesi et al. 2008)
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Xenopusyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.01% Thiomersal
      0.01% Thiomersal
      0.01% Thiomersal
    • Immunogen
      Polypeptide from the 50 kDa subunit of heparanase.
    • Purity
      98% by SDS PAGE
      98% by SDS PAGE
      98% by SDS PAGE
    • Approx. Protein Concentrations
      IgG concentration 4.0 mg/ml
      IgG concentration 4.0 mg/ml
      IgG concentration 4.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      proteoglycan metabolic process
      beta-glucuronidase activity
      carbohydrate metabolic process
      cation binding
      lysosomal membrane
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/401/40
      Western Blotting1/40001/4000
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/401/40
      Western Blotting1/40001/4000
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/401/40
      Western Blotting1/40001/4000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      OBT1975X detects bands of approximately 50kDa (corresponding to the subunit containing the active site) and 65kDa (the precursor of heparanase-1) when purified recombinant human heparanase samples are used.
    • Western Blotting
      OBT1975X detects bands of approximately 50kDa (corresponding to the subunit containing the active site) and 65kDa (the precursor of heparanase-1) when purified recombinant human heparanase samples are used.
    • Western Blotting
      OBT1975 detects bands of approximately 50kDa (corresponding to the subunit containing the active site) and 65kDa (the precursor of heparanase-1) when purified recombinant human heparanase samples are used.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Heparanase-1 Antibody Formats

    Formats Clone Applications Sizes available
    Heparanase-1 Antibody : Purified HP3/17 P, WB 0.1 mg | 0.15 mg | 50 µg
    • Copyright © 2016 Bio-Rad

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              References

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