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Hemoglobin Subunit zeta antibody | 3C4-1D5

Mouse anti Human Hemoglobin Subunit zeta:Preservative Free

Product Type
Monoclonal Antibody
Clone
3C4-1D5
Isotype
IgG1
Specificity
Hemoglobin Subunit zeta

Product Code Applications Pack Size List Price Your Price Qty
MCA3161Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader

Mouse anti Hemoglobin subunit zeta antibody, clone 3C4-1D5 recognizes the human embryonic hemoglobin zeta chain, also known as Zeta-globin, HBAZ or Hb-ζ. Hemoglobin zeta is a 141 amino acid ~14 kDa alpha-type hemoglobin chain present in early embryonic erythrocytes, forming part of the active tetrameric oxygen binding hemoglobin units Gower-1 (ζ2:γ2) Portland-1 (ζ2:ε2) and Portland-2 (ζ2:β2). Gower-2 hemoglobin does not contain any zeta components (α2:ε2) (Manning et al. 2007). Portland-2 hemoglobin containing 2ζ chains is associated with homozygous alpha thalassemia (Li et al. 2010).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-143 of human Hemoglobin subunit zeta.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
HGZ
RRID
AB_2232734
UniProt
P02008
Entrez Gene
HBZ
GO Terms
GO:0005344 oxygen transporter activity
GO:0005833 hemoglobin complex
GO:0019825 oxygen binding
GO:0020037 heme binding

MCA3161Z

020315 070115 160315

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